Journal: Cell
Article Title: Inflammation switches the chemoattractant requirements for naive lymphocyte entry into lymph nodes
doi: 10.1016/j.cell.2024.11.031
Figure Lengend Snippet: (A) IFM of naive pLN (left) or pLN 24 h post-footpad LCMV infection (right). Sections stained for PNAd+ HEVs, IgD, and CCL21. Scale bars, 100 μm. (B) Zoomed-in views of HEVs in naive (top) and inflamed (bottom) LNs. Scale bars, 20 μm. (C) RT-qPCR for Ccl21 in LNs (draining popliteal, sub-draining inguinal, or non-draining brachial) that were responding to either saline or LCMV. Each dot represents a mouse LN pair. (D) RNA-scope images of Ch25h and Cyp7b1 (red) in LNs co-stained for IgD (brown). Images are representative of at least 3 mice. Scale bars, 100 μm. (E) RT-qPCR for Ch25h and Cyp7b1 in sorted pLN HEVs, BECs, FRCs, or DN stromal cells. (F) RT-qPCR for Ch25h in LNs as in (C). (G) RT-qPCR for Ch25h and Ccl21 in LN stromal cells sorted from LCMV-draining pLNs or non-draining bLNs. Each dot represents pooled sorted cells from 6 mice in (E) and (G). All statistical tests are two-tailed unpaired t tests. * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant ( p > 0.05). Means ± standard deviation indicated in summary graphs. See also .
Article Snippet: Mouse CCL21 (6Ckine) Recombinant Protein , PeproTech , Cat# 250–13-20UG.
Techniques: Infection, Staining, Quantitative RT-PCR, Saline, RNAscope, Two Tailed Test, Standard Deviation